Additional file 3: of Microbiome–host systems interactions: protective effects of propionate upon the blood–brain barrier

Figure S2. Protective effects of propionate against LPS-induced barrier disruption in primary human brain microvascular endothelial cells (HBMEC). (a) Assessment of the paracellular permeability of HBMEC monolayers to 70 kDa FITC–dextran following treatment for 24 h with 1 μM propionate, with or without inclusion of 50 ng/ml LPS for the last 12 h of incubation; data are mean ± SEM, n = 3 independent experiments. (b) Trans-endothelial electrical resistance of HBMEC monolayers following treatment for 24 h with 1 μM propionate, with or without inclusion of 50 ng/ml LPS for the last 12 h of incubation; data are mean ± SEM, n = 3 independent experiments. (PDF 321 kb