IF analysis of macrophage infiltration in the arteries of mini-pigs with induced atherosclerosis.

<p>The macrophage content of the plaques was detected by immunofluorescence (IF) using CD68, inducible nitric oxide synthase (iNOS; M1), and arginase-1 (Arg1; M2) antibodies. A) Representative images of M1/M2 macrophage immunofluorescence in the mini-pig artery of the saline, HMGB1, and TNF-α groups (n = 4 vessels/group) (amplification 400×). B) Measurement of the co-expression area of CD68-iNOS. C) Measurement of the co-expression area of CD68-Arg1. Relative area measurements were determined using a Zeiss LSM 700. Scale bars represent 100 μm. Data are represented as the mean±SEM. *p<0.05, compared with the saline group.