Experimental setup to analyze GBM migration in 3D co-culture with stromal cells.

<p>(A). Cell populations are expanded in 2D liquid culture, recovered, fluorescently labeled and homogenously encapsulated within a 3D matrix formed by polymerization of an oligomer collagen-hyaluronan solution. (B). The position of individual glioblastoma cells (green) is tracked by confocal microscopy every 1.5 h during 15 h. (C). The trajectories of GBM single cells are analyzed to obtain accumulated migration distance, net displacement, migration velocity and directionality. Z-stack: 200 μM, step: 12 μM. Bar 100 μM.