Additional file 3. Curves of RT-qPCR for the detection of the hax1 transcript in T. reesei QM6a. T. reesei QM6a was cultivated on 1% d-glucose (brown) or without carbon source (blue). The qPCR was performed with primer pair up-hax1 for_2 and hax1 rev 1.Intron (compare Fig. 2c, PCR 2). Analysis was performed in technical triplicates. A no template control (red) and a negative control (yellow) were included in the run. Measured fluorescence is plotted against the number PCR cycles
Additional file 12: Figure S15. PCR Verification of rfp-tpho84-1, rfp-tpho84-2 mutants. Lane M, DNA ...
Additional file 4. Transcriptional levels of the major cellulase genes response to Avicel. (A) cbh1,...
Additional file 7. Western blot analysis of BGL3I-his overexpressed in T. reesei QmU2–3 using consti...
Additional file 6. RT-qPCR of the three hax1 versions in QM9414 disruption strains. Analysis of the ...
Additional file 5. RNA stability of the three HAX1 versions. The T. reesei strains QM6a (blue), QM94...
Additional file 1. Transcript levels of the neighbouring genes of the targeted site for amdS integra...
Additional file 4. Alignment of the hax1 locus of the investigated T. reesei strains. The hax1 locus...
Abstract Background Due to its capability to secrete large quantities of plant biomass degrading enz...
Additional file 3. Quantitative PCR analysis of cellulase and hemi-cellulase gene expression levels....
Additional file 1: Figure S1. T. reesei SP4 is carbon catabolite-repressed. a Growth of T. reesei SP...
Additional file 2. Codon usage of the predicted hax1 gene. For each of the 64 codons potentially mak...
Recent demands for the production of biofuels from lignocellulose led to an increased interest in en...
Additional file 1: Figure S1. Growth analysis of vib1 mutant strains on AZCL-HE-Cellulose. The image...
Additional file 5. Analysis of transcription levels of transcription factors. (A) transcription fact...
Additional file 5: Figure S5. Cellulase activity and biomass formation of QM6acre1-96 and QM6acre1-9...
Additional file 12: Figure S15. PCR Verification of rfp-tpho84-1, rfp-tpho84-2 mutants. Lane M, DNA ...
Additional file 4. Transcriptional levels of the major cellulase genes response to Avicel. (A) cbh1,...
Additional file 7. Western blot analysis of BGL3I-his overexpressed in T. reesei QmU2–3 using consti...
Additional file 6. RT-qPCR of the three hax1 versions in QM9414 disruption strains. Analysis of the ...
Additional file 5. RNA stability of the three HAX1 versions. The T. reesei strains QM6a (blue), QM94...
Additional file 1. Transcript levels of the neighbouring genes of the targeted site for amdS integra...
Additional file 4. Alignment of the hax1 locus of the investigated T. reesei strains. The hax1 locus...
Abstract Background Due to its capability to secrete large quantities of plant biomass degrading enz...
Additional file 3. Quantitative PCR analysis of cellulase and hemi-cellulase gene expression levels....
Additional file 1: Figure S1. T. reesei SP4 is carbon catabolite-repressed. a Growth of T. reesei SP...
Additional file 2. Codon usage of the predicted hax1 gene. For each of the 64 codons potentially mak...
Recent demands for the production of biofuels from lignocellulose led to an increased interest in en...
Additional file 1: Figure S1. Growth analysis of vib1 mutant strains on AZCL-HE-Cellulose. The image...
Additional file 5. Analysis of transcription levels of transcription factors. (A) transcription fact...
Additional file 5: Figure S5. Cellulase activity and biomass formation of QM6acre1-96 and QM6acre1-9...
Additional file 12: Figure S15. PCR Verification of rfp-tpho84-1, rfp-tpho84-2 mutants. Lane M, DNA ...
Additional file 4. Transcriptional levels of the major cellulase genes response to Avicel. (A) cbh1,...
Additional file 7. Western blot analysis of BGL3I-his overexpressed in T. reesei QmU2–3 using consti...