Study on the effect of FGFR4 gene silencing on biological properties of hela cervical cancer cells

Objective: To discuss the the effect of FGFR4 gene silencing on growth, metastasis and the cis-platinum (DDP) chemotherapy sensitivity of Hela cervical cancer cells. Methods: The Hela cervical cancer cells were cultured and divided into 5 groups: blank control group, negative control group, FGFR4-siRNA group, DDP group and FGFR4-siRNA+DDP group. The siRNA targeted to FGFR4 was constructed and transfected into the cells in FGFR4-siRNA group and FGFR4-siRNA+DDP group, and DDP (10 毺 g/mL) was added into the DDP group and FGFR4-siRNA+DDP group. The growth inhibition ratio was detected by MTT and the apoptosis rate was detected by FCM after 24 h, 48 h and 72 h culturing respectively. The mRNA and protein expression level of FGFR4, VEGF and MMP2 were detected by RT-PCR and western blot respectively. Results: Compared with the blank control, the proliferative activity of FGFR4-siRNA group decreased after the FGFR4 being silenced, and the growth inhibition ratio and apoptosis rate increased, the FGFR4 did almost not express and the expression level of VEGF and MMP2 decreased.The growth inhibition ratio and apoptosis rate of FGFR4-siRNA+DDP group were higher than that of FGFR4-siRNA group and DDP group, the FGFR4 did almost not express either and the the expression level of VEGF and MMP2 were lower than the FGFR4-siRNA group and DDP group. Conclusion: It can be obtained that silencing the FGFR4 of cervical cancer could inhibit cell growth, promote cell apoptosis and increase the chemosensitivity, down-regulate the expression of VEGF and MMP2 to inhibit the ability of invasion and metastasis, which could be one of the targeted therapeutic targets of cervical cancer