Simulated transformation.

<p>A) The mRNA level of each gene is altered 10-fold and EGF + insulin response of starved cells for 24 hours is simulated. Gray bar denotes wild-type, dots denote tumor suppressors (10-fold reduction), and no label denote proto-oncogenes (10-fold increase). B-D) MAP2K2 overexpression (OE-left), wild-type (middle), and MEK-Raf high affinity (right, unphosphorylated MEK has 10-fold higher affinity for all Raf species) were simulated as above and either the level of Raf bound to MEK at t = 0 (B), % proliferating cells (C) or time-averaged ppERK levels (D) were calculated.