The slicing activity of CSR-1 is required to inhibit EGA.

<p>(A) Detection of EGA transcripts by RT-qPCR in gonads from animals of the indicated genotypes. The <i>csr-1(tm892)</i> animals were expressing either wild-type CSR-1 (CSR-1^WT), or a CSR-1 variant with mutations compromising its slicing activity (CSR-1^SIN); only the gonads from the latter strain expressed embryonic genes. Each bar represents the mean of three independent biological replicates. Error bars and p-values indicated as explained before. (B) Among a group of 446 early-expressed embryonic genes, only 30 are potentially targeted by CSR-1, based on the presence of antisense 22G RNAs associated with CSR-1.