Dystrophin expression in different muscle types obtained from paediatric controls.

<p>Ten muscle blocks derived from different muscle groups obtained from seven controls (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194540#pone.0194540.t001" target="_blank">Table 1</a>) were analysed performing anti-dystrophin ab15277/anti-spectrin and anti-dystrophin MANDYS106/anti-laminin stainings. Entire muscle sections were acquired by the Axio Scan slide scanner and processed by the Definiens algorithm, exploiting spectrin or laminin staining for fibre identification. Dystrophin was quantified in each individual fibre using either anti-dystrophin ab15277 (Fig 4a) or MANDYS106 (Fig 4b) intensities plotted as arbitrary units. We acquired a different number of myofibres (22,000 fibres for control 3 to 5,000 fibres for control 5) per section depending on the cross sectional area.