Localization of TcCHT7 protein in adult cuticle.

<p>Immunohistochemical analysis was performed to determine the locations of TcCHT7 in adult elytral (A) and ventral abdominal (B) cuticles. Cryosections of pharate adults (5 d-old pupae) that had been injected with ds<i>TcCHT7</i> or ds<i>TcVer</i> in the late larval stage were incubated with the anti-TcCHT7 antibody, which was then detected by Alexa Fluor 546-conjugated goat anti-rabbit IgG (red). Cuticular chitin and nuclei were stained with FITC-conjugated chitin-binding protein probe (green) and To-Pro-3 (blue), respectively. P, pupal cuticle; E, elytral dorsal cuticle; C, epidermal cell; H, hindwing; V, ventral abdominal cuticle. Scale bar = 20 μm.