Sequencing of the molecular barcode isolated from mosquito #27 that fed on 776301.

<p>Viral RNA that was isolated from the saliva, body, and legs of mosquito #27 at day 25 post feeding was converted into cDNA and then split into two separate PCR reactions with primer set A (178 bp) and B (131 bp). The theoretical number of cDNA molecules used in each PCR reaction is shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006964#ppat.1006964.t005" target="_blank">Table 5</a>. PCR products were tagged and sequenced. The frequency of each barcode detected was quantified. Mosquito #27 fed on 776301 on day 4, so the frequencies of barcodes detected in the plasma of 776301 from <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006964#ppat.1006964.g006" target="_blank">Fig 6</a> are shown. The frequency of any sequence in the region of the barcode that was not considered authentic is listed as ‘Other.’