Enhanced <i>ex vivo</i> recall responses of Apaf1-deficient T cells.

<p>(A and B) LN cells from OVA-immunized <i>Apaf1</i>^f/f-OTII or Lck-<i>Cre</i>-<i>Apaf1</i>^f/f-OTII mice were assessed for expression of CD69, CD44 and CD62L. Percentages (A) and absolute numbers (B) of CD69⁺ cells and CD44^highCD62L^low cells in TCR Tg-positive population (OTII population) are shown. (C) LN cells from immunized mice were re-stimulated with OVA or anti-CD3ε antibody at the indicated concentration for 48 hours. Percentages of CD69⁺ cells and CD44^highCD62L^low cells are shown. Open columns; <i>Apaf1</i>^f/f-OTII mice and closed columns; Lck-<i>Cre</i>-<i>Apaf1</i>^f/f-OTII mice. Shown are mean+SD of triplicated samples. Experiments were repeated three times with similar results. *; p<0.05, **; p<0.01, and ***; p<0.002 compared between <i>Apaf1</i>^f/f and Lck-<i>Cre</i>-<i>Apaf1</i>^f/f cells, <i>t</i>-tests.