Dynein arm subunits and interaction partners of ZMYND10 were downregulated in <i>Zmynd10</i>^{<i>−/−</i>} mice.

<p><b>(A)</b> Representative immunoblot analyses of DNAH7, DNAI2, DNAI1, IQUB, LRRC6, C21ORF59, and ZMYND10 in the testis extracts from <i>Zmynd10</i>^<i>+/+</i> and <i>Zmynd10</i>^{<i>−/−</i>} mice. <b>(B)</b> Bar graphs represent band intensities of the blot shown in panel A, and data represent the mean ± SD of more than three independent experiments. Band intensities were normalized to β-actin. *<i>P</i> < 0.05; **<i>P</i> < 0.005; t-test. <b>(C–E)</b> Immunofluorescence analysis of tracheal multiciliated epithelia in <i>Zmynd10</i>^<i>+/+</i> and <i>Zmynd10</i>^{<i>−/−</i>} mice. (C) DNAH5 (red), an ODA heavy chain protein, localized to both cilia and cytoplasm of the <i>Zmynd10</i>^<i>+/+</i> trachea, but was almost completely absent from the <i>Zmynd10</i>^{<i>−/−</i>} trachea. DNAI2 (red) was present in both the cilia and cytoplasm in the <i>Zmynd10</i>^<i>+/+</i> trachea and colocalized with acetylated α-tubulin (Ac-α-tub) along cilia. However, DNAI2 expression was negligible in the <i>Zmynd10</i>^{<i>−/−</i>} trachea (D). IQUB (red), a cytoplasmic protein and ZMYND10 interaction partner, was almost undetectable in the <i>Zmynd10</i>^{<i>−/−</i>} trachea (E). Scale bars, 10 μm. <b>(F–H)</b> Graphs comparing reads per kilobase of transcript per million mapped reads (RPKM) of dynein arm assembly factors and ZMYND10-interacting proteins in the testis (F), lung (G), and brain (H) tissues of <i>Zmynd10</i>^<i>+/+</i> and <i>Zmynd10</i>^{<i>−/−</i>} mice.