Collision energy is a key parameter determining the information content of beam-type collision induced dissociation tandem mass spectrometry (MS/MS) spectra, and its optimal choice largely affects successful peptide and protein identification in MS-based proteomics. For an MS/MS spectrum, quality of peptide match based on sequence database search, often characterized in terms of a single score, is a complex function of spectrum characteristics, and its collision energy dependence has remained largely unexplored. We carried out electrospray ionization-quadrupole-time of flight (ESI-Q-TOF)-MS/MS measurements on 2807 peptides from tryptic digests of HeLa and <i>E. coli</i> at 21 different collision energies. Agglomerative clustering of the res...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
Collision energy is a key parameter determining the information content of beam-type collision induc...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
Collision-induced dissociation product ion spectra of a series of doubly charged tryptic peptide ion...
QTof CID and Orbitrap HCD are the most commonly used fragmentation techniques in mass spectrometry b...
Rigorous validation of amino acid sequence is fundamental in the characterization of original and bi...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
Collision energy is a key parameter determining the information content of beam-type collision induc...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
International audienceCollision energy is a key parameter determining the information content of bea...
Collision-induced dissociation product ion spectra of a series of doubly charged tryptic peptide ion...
QTof CID and Orbitrap HCD are the most commonly used fragmentation techniques in mass spectrometry b...
Rigorous validation of amino acid sequence is fundamental in the characterization of original and bi...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...
International audienceRigorous validation of amino acid sequence is fundamental in the characterizat...