MOESM1 of Evolutionary engineering improves tolerance for medium-chain alcohols in Saccharomyces cerevisiae

Additional file 1: Figure S1. Representative growth curves for evolved strains in 0.15% n-hexanol. Strains were grown in 10 ml YPD + 0.15% n-hexanol in capped 50 ml culture tubes. Figure S2. Growth curves for evolved strains in 0.5% n-pentanol. Figure S3. Growth curves for evolved strains in 0.05% n-heptanol. Figure S4. Growth curves to examine the impact of Pdr5 in 0.15% n-hexanol. Figure S5. Structure of S. pombe eIF2B with the positions of alcohol-sensitive S. cerevisiae eIF2Bγ and eIF2Bβ mutations mapped. The subunit arrangement depicted is of the α2β2δ2 hexameric regulatory subcomplex bound to two γε dimeric catalytic subcomplexes on its opposite sides [40]. The α-, β-, γ-, δ- and ε-subunits are colored blue, cyan, orange, green and pink, respectively. The positions of mapped alcohol-sensitive S. cerevisiae eIF2Bγ and eIF2Bβ mutations are indicated on the eIF2Bγε subcomplex on the left only. The location of the interfaces for eIF2γ and eIF2α binding to eIF2B are indicated by the red and orange hatched boxes, respectively [40, 41]. Figure S6. Structure of S. cerevisiae eIF2α (residues 1–175) [42] indicating the phosphorylation site, S51, the alcohol-sensitive D77 (D76 in structure that lacks M1), and residues that have been shown to be important for interaction with eIF2B (E49, K79, G80, and R88) [42, 43]. Table S1. List of primers used in this study. Table S2. List of plasmids for expression of Gcd1p and Sui2p mutants