A USEFUL METHODOLOGY TO SELECT LIPASE-CATALYZED TRANSESTERIFICATION AIMING BIODIESEL APPLICATION

<p class="Estilo1"><span lang="EN-US">The application of lipases in various fields has been notably increased in the last few decades and qualitative/quantitative improvements need to be done. However, many methodologies of screening are described in order to find a good lipase producer and statistical optimization is a necessary tool to improve lipase production. In this work, an isolation of <span>filamentous fungi lipase producers </span>and a transesterification capacity screening <span>was evaluated. Four fungi were chosen to the transesterification reaction assays and the best fungus selected was submitted to a submerged fermentation. Parameters of the culture medium were optimized using response surface methodology. Selected liquid medium was SR at 30 °C, 72 h, 100 rpm. Corn oil was the best carbon source and together with Tween 80 increased two-fold the lipase activity. After the experimental design, the new medium optimized were 3.5-fold higher than the original liquid medium and was composed by 0.5% corn oil, </span><span>0.012% </span>MgSO₄.7H₂O, 0.015% KH₂PO₄, 0.05% NH₄H₂PO₄. Hence, the lipase produced proved its transesterification capacity and can be used for biodiesel production.</span>