Additional file 4: of Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity

Figure S2. Different effects of B3GALNT2 in vitro and in vivo. a Cell cycle analysis of Huh7-shB3GALNT2 and control cells. b Annexin V and PI staining of Huh7-shB3GALNT2 and control cells which show the effects of B3GALNT2 on apoptosis. c In vivo effect of B3GALNT2 overexpression in subcutaneous xenograft model at 4th week after inoculation (n = 6 in each group). Representative photos of tumor-bearing mice are shown on the left, and statistical data is shown on the right. d IHC analysis of B3GALNT2 and F4/80 levels in tumor tissues from xenografts. Cell count of macrophages is shown on the right. e Representative IHC staining of B3GALNT2, CD68, and CD206 in human HCC tumor tissues. f Correlation between B3GALNT2 and CD206 levels in human HCC cohort. (TIFF 5233 kb