Subcellular localization of IP₃K-A and increased number of synaptic vesicles in IP₃K-A Tg mice.

<p>(A), Distribution pattern of IP₃K-A in the adult mouse brain subcellular fractions. PSD-95 and synaptophysin (SynPhy) were used as controls. H, homogenates; P1, nuclei and large debris; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. (B), Representative EM images of hippocampal CA1 synapses in WT and Tg mice. Scale bar: 100 mm. (C), Quantification of the number of synaptic vesicles per area (WT, n = 60 synapses, 3 mice; Tg, n = 60, 3). Presynaptic terminals with well-defined postsynaptic density (arrows) were chosen for analysis. Dashed lines indicated the presynaptic area for the analysis of synaptic vesicle (arrowheads) density per area.