Macrophage morphology analysis.

<p>(A) Unstimulated isolated monocytes after 24 hours of incubation, partly apoptotic. (B) Unstimulated MDM, i.e. monocytes stimulated with M-CSF, after 24 hours of incubation; different phenotypes of macrophages were identified as dendritiform (C), round (D), and elongated (E). (F, G) Individual donors revealed distinct distribution patterns for all three macrophage phenotypes (only round phenotype presented, median of all stimuli at 24 and 48 hours). (H) Increase or decrease of round macrophages in percent in different stimulation conditions (lipopolysaccharide [LPS], interferon gamma [IFNγ]), fragment-free supernatant fluid phase [FF], small [SF], and large corneal fragments [LF]) relative change to cell counts in unstimulated samples at 24 hours of incubation (pooled data from 7 individual experiments with individual donors as in Fig 1F).