Effects of length of hiPSC differentiation on force production.

<p>Data shown as mean ± SEM. A. Representative hiPSC-CMs from day 14, day 30, and day 90 post-differentiation, and representative NRVM on substrate with modulus 9.8 kPa. B. Total force versus length of differentiation (mean = 0.012 ± 0.001 μN, n = 17; 0.083 ± 0.013 μN, n = 15; 0.103 ± 0.011 μN, n = 24; 0.113 ± 0.016 μN, n = 12). C. Cell area versus length of differentiation (mean = 605.7 ± 47.1 μm², 702.3 ± 63.2 μm², 898.2 ± 64.4 μm², 741.9 ± 61.4 μm²). D. Normalized force versus length of differentiation in culture (mean = 2.12 ± 0.23 mN/mm2 x 10⁻⁵, 13.0 ± 2.14 mN/mm2 x 10⁻⁵, 11.4 ± 1.4 mN/mm2 x 10⁻⁵, 15.3 ± 1.5 mN/mm2 x 10⁻⁵).