Effects of miR-29b on the proliferation and apoptosis of CL cells.

<p>(A) Bcl-2 was up-regulated and Bax was down-regulated in CL cells transfected with a miR-29b mimic. (B) The ratio of Bax to Bcl-2 was reduced in CL cells transfected with miR-29b mimic. (C) Bcl-2 and Bax protein expression in CL cells. (D) Relative gray value analysis of Bax and Bcl-2 protein expression. (E) and (F) Flow cytometry was performed to observe the apoptosis of CL cells via staining with Annexin-V-FLUOS/PI. (G) Apoptosis rates of CL cells after transfection with miR-29b mimic or NC. (H) Analysis of the viability of CL cells using the CCK-8 method. All data are presented as the means ± standard deviation from at least three independent experiments. Statistical significance was determined via one-way ANOVA. The same letters indicate that the difference is not significant, and different letters (a, b and c) indicate that the difference is significant. P < 0.05 was considered significant. * P < 0.05, ** P < 0.01.