Additional file 9: of Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates

Figure S5. Lactobacillus genera are quantitatively increased in the gut of synbiotic-supplemented juveniles. DNA was isolated from anal swabs and stool samples collected at pre-, mid-, post-supplementation. PCR was performed to detect Lactobacillus at the genus level. (A) While bacterial DNA from probiotics was undetectable at the species level in the gut of supplemented juveniles, we were able to detect Lactobacillus in the gut at the genus level in both pre-, mid, and post supplemented juveniles. (B) Increases in Lactobacillus at the genus level was detectable by quantitative real-time PCR (qPCR). (C) The majority of supplemented juveniles (5/7) had a detectable increase in Lactobacillus at the genus level by qPCR, which resolved post-supplementation. n = 7 subjects per time point. (PDF 5089 kb