The assessment of chondrogenesis following the application of CGA and alginate.

<p>(<b>A</b>) Sketches illustrating chondrocytes isolated from the embryonic 15-day chick articular cartilage in alginate with 60μM CGA. (<b>B-D)</b> H&E staining on the 21-day culture with alginate only (B), chondrocytes + alginate (C) or chondrocytes + alginate + CGA (D). (<b>B1-D1)</b> High magnification images from B-D respectively. <b>(E-F)</b> Safranin-O staining on 21-day cultured chondrocytes + alginate (E) or chondrocytes + alginate + CGA (F). (<b>E1-F1)</b> High magnification images from E-F respectively. (<b>G)</b> Bar chart showing the comparison of IOD from Safranin-O staining between controls and CGA groups. Scale bars = 500μm in B-F and 100μm in B1-F1.