Urea-induced unfolding of rRsbW.

<p>(A) The ellipticity values of rRsbW at 222 nm (θ₂₂₂), were derived from their far-UV CD spectra (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0195416#pone.0195416.s001" target="_blank">S1 Fig</a>), normalized (Nor), and plotted versus the corresponding urea concentrations. The ANS fluorescence intensity values at 480 nm (B) and the intrinsic Tyr fluorescence intensity values at 308 nm (C) were obtained from the corresponding spectra of rRsbW (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0195416#pone.0195416.s001" target="_blank">S1 Fig</a>) were plotted similarly.