Intramembranous bone formation after callus distraction is augmented by increasing axial compressive strain - Fig 2

<p><b>a-b. Experimental set up <i>in vivo</i> and <i>ex vivo</i>.</b> The <i>in vivo</i> experiment (Fig 2A) consisted of two phases of manipulations (grey areas). Following surgery and latency phase, the animals were subjected to ten days of lateral callus distraction creating a gap of regenerating tissue between tibial cortex and titanium plate (hatched area in X-ray). Seven days into maturation, the regenerating tissue received electromechanically induced cyclic compression of two differing amplitudes and frequencies (Group 0.1 is depicted by the black line; Group 0.6 is depicted by the dotted line). The <i>ex vivo</i> analysis was performed by μCT, histology and immunohistochemistry (Fig 2B). Volume of interest (VOI) dimensions for μCT analysis are shown using exemplary sagittal and transaxial images.