Prevalence of respiratory pathogens detected in dogs with kennel cough in Poland

Kennel cough is a multifactorial disease occurring all over the world; however, its epidemiology is still not fully understood. To the authors’ knowledge, no studies monitoring the occurrence of infectious agents responsible for kennel cough have been carried out in Poland. Therefore, the objective of our study was to determine which of the four pathogens most frequently isolated in other countries are predominant in north-eastern Poland. Swabs from the upper respiratory tract and tracheal lavage fluids from dogs (n = 40) exhibiting symptoms of this disease were analysed. Canine herpesvirus, canine parainfluenza virus, canine adenovirus type 2 and Bordetella bronchiseptica were identified by polymerase chain reaction. At least one of the above-listed infectious agents was found in all dogs. The predominant pathogen within the area under our study, both in mono- and co-infections, was canine herpesvirus (32/40), whereas canine adenovirus type 2 occurred least frequently (4/40). The effectiveness of detection of selected pathogens from both types of study material was also compared. Tracheal lavage fluid was more suitable for the isolation of canine herpes virus, canine parainfluenza virus, and Bordetella bronchiseptica. Swabs from the upper respiratory tract were more suitable for the isolation of canine adenovirus type 2