Liver Melanomacrophages and Gluthation S-Transferase Activity in Leptodactylus chaquensis (ANURA, LEPTODACTYLIDAE) as Biomarkers of Oxidative Stress Due to Chlorpyrifos Exposition

Ivan Huespe
Mariana Cabagna-Zenklusen
Lucila Marilén Curi
Paola Peltzer
Maximilano Andres Attademo
Noelia Villafañe
Rafael Carlos Lajmanovich

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Publication date

May 2017

Publisher

Universidad Nacional de Colombia

Journal

issn:1900-1649

Abstract

We quantified and compared the hepatic melanomacrophage (MM) and glutathione S-transferase (GST) enzyme activity (two oxidative stress biomarkers) in the liver of Leptodatylus chaquensis adults (Anura, Leptodactylidae) collected in a rice field (CA) in San Javier department, Santa Fe (Argentina), seven days after the application of chlorpyrifos and in a reference site (SR). The histological analysis revealed a significant amount (p = 0.028) and area occupied by MM (p = 0.017) in livers of CA compared to SR. Furthermore, a significant inhibition of GST activity was recorded in the CA frogs compared to the SR (p = 0.030). The histopathological and enzymatic effects provide evidences of ecotoxicological risk for anurans in rice field with CPF ...