Pathomorphology of rat brain meninges in experimental subarachnoid hemorrhage

Aim. To study pathomorphological changes and vascular reactions of meninges and influence of nitric oxide donators on it in subarachnoid hemorrhage. Methods and results. Eighteen white rats underwent suboccipital injection of autologous arterial blood to create model of subarachnoid hemorrhage (SAH) and study microanatomy of brain meninges stained with hematoxylin-eosin and metylen blue-basic fuchsine. Disorientation of dural collagen microfibers, infiltration with leukocytes, irregularity of connective fibers structure and tissue cell apoptosis were noted. In animals with SAH after treatment with donor of nitric oxide L-arginin Tivortin on background of arteriolar smooth muscle layer edema order of myocytes nuclei in accordance with their spiral orientation in thickened media was noted, spindly nuclei of endothelial cells are normochromic, located evenly along basal membranes of arterioles, apoptosis phenomenon in micropreparations does not occur. Conclusion. This shows that nitric oxide donator L-arginin injections provide reduction of inflammatory, necrobiotic processes and cell apoptosis of brain membranes and arterioles in the experimental SAH