Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment

In this study, a novel tetrapeptide hydrophilic interaction chromatography (HILIC) material was synthesized, and corresponding enrichment method for glycopeptides was developed. The tetrapeptide modified silica gel materials (denoted as Poly. DAPD) were synthesized by atom. transfer radical. polymerization (ATRP) and characterized by N-2 adsorption desorption and thermometer, thermal gravimetric analyzer (TGA) and X. ray photoelectron spectrometer (XPS). The characterization results indicated that tetrapeptide had been successfully synthesized on silica gel. Poly. DAPD materials showed high enrichment selectivity toward fetuin glycopeptides under solid. phase extraction (SPE) mode. Comparing with commercialized ZIC. HILIC in glycopeptides enrichment of fetuin digest which mixed with 5 mole ratio of albumin bovine (BSA), the as. prepared materials showed higher selectivity in both aspects of the identified number of glycopeptides and anti. interference property. The SPE results demonstrated that the tetrapeptide. based HILIC materials could be a potential tool in large. scale glycosylation analysis