Simultaneous fMRI with GCaMP6-mediated neuronal and astrocytic calcium signal recording

Neurovascular coupling is the basis of the BOLD fMRI, however, the mechanisms of the neurovascular coupling remains elusive. By simultaneous cell-type specific Ca2+ recording with BOLD fMRI, it allowed us to study the cellular specific coupling events through the neuron-glia-vessel network. This work showed neuronal Ca2+ and evoked astrocytic Ca2+ signal were positively correlated to the fMRI signal, but an intrinsic astrocytic Ca2+ signal was negatively correlated to the fMRI signal in the cortex. It indicated a novel neuron-glia-vascular coupling event mediated through the intrinsic astrocytic calcium signal (details mechanistic study in another abstract: ID 4475)