The Determination of Prolactin Gene Polymorphism Using PCR-RFLP Method within Indigenous Anatolian Water Buffalo and Brown Swiss

The objective of this research was to determine the prolactin gene polymorphism within Indigenous Anatolian Water Buffalo breed and Brown Swiss cattle by using PCR-RFLP method. Experimental material for this study consists of 45 Indigenous Anatolian Water Buffalo breed and 30 Brown Swiss cattle. According to the research evidence, Indigenous Anatolian Water Buffalo breed was monomorphic at the exon 3 PRL-RsaI loci. On the other hand, the polymorphism association with exon 3 PRL-RsaI loci detected in Brown Swiss cattle. The allelic frequencies (A, B) in Brown Swiss cattle were 0.82 and 0.18, respectively. The genotype frequencies of AA and AB were 0.63 and 0.37, respectively. The BB genotype was not found in the present study