Defining ecological and chemical reference conditions and restoration targets for nine European lakes

This paper aims to determine the ecological and chemical reference conditions (~1800–1850 AD) and degree of floristic change at nine enriched lakes, covering a range of types across Europe, using fossil diatom assemblages in dated sediment cores and application of total phosphorus (TP) transfer functions. Additionally the study assesses the potential of analogue matching as a technique for identifying reference sites and for estimating reference TP concentrations for the study lakes using a training set of 347 European lakes and 719 diatom taxa. Oligotrophic, acidophilous to circumneutral taxa were predominant in the reference samples of several of the deep lakes, and benthic Fragilaria spp. dominated the reference samples of two high alkalinity shallow lakes. The degree of floristic change from the reference sample, assessed using the squared chord distance (SCD) dissimilarity coefficient, revealed that two sites had experienced slight change (Lago Maggiore, Felbrigg Lake), five experienced moderate change (Mjoesa, Loch Davan, Loch Leven, White Lough, Esthwaite Water), and two showed evidence of major change (Groby Pool, Piburger See). For three lakes, there were no analogues in the diatom dataset owing to the uniqueness and diversity of the diatom reference assemblages. For the remaining six sites the number of analogues ranged from 2 to 44. For two deep lakes most of the analogues seemed appropriate as they were of the same type and had low TP concentrations. However, for two other deep lakes and two shallow lakes some of the analogues differed markedly in their depth and alkalinity from the lake in question or had TP concentrations seemingly too high to represent reference conditions suggesting that the analogues may not be suitable as reference sites. For the deep lakes, similar reference TP values were calculated using the EDDI Combined TP transfer function and the analogue matching technique with concentrations typically