Methanothermobacter thermoautotrophicus RNA ligase (MthRnl) catalyzes formation of phosphodiester bonds between the 5′-phosphate and 3′-hydroxyl termini of single-stranded RNAs. It can also react with RNA with a 3′-phosphate end to generate a 2′,3′-cyclic phosphate. Here, we show that MthRnl can additionally remove adenosine from the 3′-terminus of the RNA to produce 3′-deadenylated RNA, RNA(3′-rA). This 3′-deadenylation activity is metal-dependent and requires a 2′-hydroxyl at both the terminal adenosine and the penultimate nucleoside. Residues that contact the ATP/AMP in the MthRnl crystal structures are essential for the 3′-deadenylation activity, suggesting that 3′-adenosine may occupy the ATP-binding pocket. The 3′-end of cleaved RNA(3...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
An ATP-dependent RNA ligase from Methanobacterium thermoautotrophicum (MthRnl) catalyzes intramolecu...
RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this...
The RNA ligase RtcB is conserved in all domains of life and is essential for tRNA maturation in arch...
SummaryRNA 3′-phosphate cyclase (RtcA) synthesizes RNA 2′,3′ cyclic phosphate ends via three steps: ...
AbstractBackground: The protein enzymes RNA ligase and DNA ligase catalyze the ligation of nucleic a...
SummaryRNA 3′-phosphate cyclase (RtcA) synthesizes RNA 2′,3′ cyclic phosphate ends via three steps: ...
AbstractProcesses involved in RNA metabolism can be distinguished by the nature of the sugar phospha...
The 3\u27 end cleavage/polyadenylation (3\u27 processing) is important in generating a functional me...
RNA 3′-phosphate cyclase (RtcA) catalyzes the ATP-dependent cyclization of a 3′-phosphate to form a ...
Polynucleotide phosphorylase (PNPase), an enzyme conserved in bacteria and eukaryotic organelles, pr...
SummaryT4 RNA ligase 2 (Rnl2) and kinetoplastid RNA editing ligases exemplify a family of RNA repair...
The 3\u27 end cleavage/polyadenylation (3\u27 processing) is important in generating a functional me...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
An ATP-dependent RNA ligase from Methanobacterium thermoautotrophicum (MthRnl) catalyzes intramolecu...
RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this...
The RNA ligase RtcB is conserved in all domains of life and is essential for tRNA maturation in arch...
SummaryRNA 3′-phosphate cyclase (RtcA) synthesizes RNA 2′,3′ cyclic phosphate ends via three steps: ...
AbstractBackground: The protein enzymes RNA ligase and DNA ligase catalyze the ligation of nucleic a...
SummaryRNA 3′-phosphate cyclase (RtcA) synthesizes RNA 2′,3′ cyclic phosphate ends via three steps: ...
AbstractProcesses involved in RNA metabolism can be distinguished by the nature of the sugar phospha...
The 3\u27 end cleavage/polyadenylation (3\u27 processing) is important in generating a functional me...
RNA 3′-phosphate cyclase (RtcA) catalyzes the ATP-dependent cyclization of a 3′-phosphate to form a ...
Polynucleotide phosphorylase (PNPase), an enzyme conserved in bacteria and eukaryotic organelles, pr...
SummaryT4 RNA ligase 2 (Rnl2) and kinetoplastid RNA editing ligases exemplify a family of RNA repair...
The 3\u27 end cleavage/polyadenylation (3\u27 processing) is important in generating a functional me...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...
Cellular RNA polymerases RNAPs can become trapped on DNA or RNA, threatening genome stability and...