O-(2-[18F-]fluoroethyl)-L-tyrosine PET in gliomas: influence of data processing in different centres

Background: PET using O-(2-[F-18] fluoroethyl)-L-tyrosine (F-18-FET) is an established method for brain tumour diagnostics, but data processing varies in different centres. This study analyses the influence of methodological differences between two centres for tumour characterization with F-18-FET PET using the same PET scanner. Methodological differences between centres A and B in the evaluation of F-18-FET PET data were identified for (1) framing of PET dynamic data, (2) data reconstruction, (3) cut-off values for tumour delineation to determine tumour-to-brain ratios (TBR) and tumour volume (Tvol) and (4) ROI definition to determine time activity curves (TACs) in the tumour. Based on the F-18-FET PET data of 40 patients with untreated cerebral gliomas (20 WHO grade II, 10 WHO grade III, 10 WHO grade IV), the effect of different data processing in the two centres on TBRmean, TBRmax, T-vol, time-to-peak (TTP) and slope of the TAC was compared. Further, the effect on tumour grading was evaluated by ROC analysis. Results: Significant differences between centres A and B were found especially for TBRmax (2.84 +/- 0.99 versus 3.34 +/- 1.13; p <0.001), T-vol (1.14 +/- 1.28 versus 1.51 +/- 1.44; p <0.001) and TTP (22.4 +/- 8.3 min versus 30.8 +/- 6.3 min; p