Lipid droplet dynamics and insulin sensitivity upon a 5-day high-fat diet in Caucasians and South Asians
- Gemmink, Anne
- Bakker, Leontine E. H.
- Guigas, Bruno
- Kornips, Esther
- Schaart, Gert
- Meinders, A. Edo
- Jazet, Ingrid M.
- Hesselink, Matthijs K. C.
DOIAbstract
A 5-day High-Fat High-Calorie diet (HFHC-diet) reduces insulin-stimulated glucose disposal (Rd) in South Asian, but not Caucasian healthy lean males. We aimed to investigate if differences in myocellular lipid handling are underlying this differential response. A two-step hyperinsulinemic-euglycemic clamp and muscle biopsies were performed in 12 healthy lean Caucasian and South Asian males (BMI <25 kg/m(2), 19-25 years) before and after a 5-day HFHC-diet (regular diet + 375 mL cream/day; 1275 kcal/day; 94% fat). Triglyceride extractions and Western Blots for lipid droplet and mitochondrial proteins were performed. Intramyocellular lipid content and HFHC-diet response were similar between ethnicities (group effect: P = 0.094; diet effect: +s...
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