Time course of induction of mRNA expression of tumor necrosis factor (TNF)-α, IL-1β, IL-6, IL-8, and cyclooxygenase 2 (COX-2)

<p><b>Copyright information:</b></p><p>Taken from "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor"</p><p>Arthritis Research & Therapy 2004;6(4):R384-R392.</p><p>Published online 21 Jun 2004</p><p>PMCID:PMC464924.</p><p>Copyright © 2004 Westra et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> Monocyte-derived macrophages from healthy controls (= 2) were stimulated for increasing periods of time with lipopolysaccharide (50 ng/ml). mRNA expression was determined with real-time RT-PCR and results were calculated as fold induction in comparison with unstimulated cells (fold induction = 1)