Reconstitution of 3′-PG processing and subsequent gap filling in SCAN1 extracts by recombinant TDP1

<p><b>Copyright information:</b></p><p>Taken from "Deficiency in 3′-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1)"</p><p>Nucleic Acids Research 2005;33(1):289-297.</p><p>Published online 12 Jan 2005</p><p>PMCID:PMC546157.</p><p>© 2005, the authors © </p> The plasmid substrate was treated with 3 mg/ml SCAN1 whole-cell extract (subject 1635) and analyzed as in . In some cases, 25 ng of recombinant TDP1 and/or 200 ng of XRCC4/DNA ligase IV complex (Trevigen) were added. Some samples contained 5 mM EDTA instead of 0.5 mM MgCl, as indicated