Effect of cadmium on the ATP hydrolysis and ATP-binding activities of MSH2–MSH6

<p><b>Copyright information:</b></p><p>Taken from "Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2–MSH6 complex"</p><p>Nucleic Acids Research 2005;33(4):1410-1419.</p><p>Published online 3 Mar 2005</p><p>PMCID:PMC552968.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> MSH2–MSH6 was pre-incubated with Cd (0–0.5 mM) at 4°C for 10 min. Aliquots were withdrawn and assayed for ATP hydrolysis (black bars) and ATP binding (white bars) as described in Materials and Methods. Results are expressed as a percentage of the activity of MSH2–MSH6 in the absence of Cd. For ATP hydrolysis 100% corresponds to 200 pmol ATP hydrolyzed, while for ATP binding, 100% of the activity corresponds to 2 pmol ATP bound. The average of three experiments for each concentration of Cd is presented. Standard deviation is included