Primary cortical cells isolated from 18–20 day old fetal rat brains were cultured for 7 days and treated with 1 mM glutamate for 18 h

<p><b>Copyright information:</b></p><p>Taken from "Glutamate-induced apoptosis in primary cortical neurons is inhibited by equine estrogens via down-regulation of caspase-3 and prevention of mitochondrial cytochrome c release"</p><p>BMC Neuroscience 2005;6():13-13.</p><p>Published online 24 Feb 2005</p><p>PMCID:PMC555946.</p><p>Copyright © 2005 Zhang and Bhavnani; licensee BioMed Central Ltd.</p> The cells were processed as described under "Methods". The isolated DNA was electrophoresed on TBE agarose gel for 1.5 h at 100 V. The DNA fragments were visualized by staining with ethidium bromide. Lane 1 = 1 kb DNA ladder; lane 2 = DNA from untreated cortical cells prior to culture; lane 3 = DNA from untreated control cells cultured for 1 day; lane 4 = DNA from untreated control cells cultured for 2 days; lane 5 = DNA from cells cultured for 2 days and treated with glutamate for 18 h; lane 6 = DNA from untreated control cells cultured for 7 days; lane 7 = DNA from cells cultured for 7 days and then treated for 18 h with glutamate. Note the potentiation of DNA fragmentation in presence of glutamate