Semi-quantitative RT–PCR of endogenous and transgene transcripts in wild-type and transplastomic shoots

<p><b>Copyright information:</b></p><p>Taken from "Expression of complementary RNA from chloroplast transgenes affects editing efficiency of transgene and endogenous chloroplast transcripts"</p><p>Nucleic Acids Research 2005;33(5):1454-1464.</p><p>Published online 8 Mar 2005</p><p>PMCID:PMC1062874.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Endogenous (upper panel) and transgene transcripts (lower panel) were amplified by reverse transcription followed by either 25 or 40 cycles of PCR. Products were separated on an agarose gel and stained with ethidium bromide. The faint band in the control WT lane is primer dimer