Dose response of IL-10 inhibition of interferon gamma (IFN-γ) production by CD4T cells after CD3 and CD28 costimulation in patients with rheumatoid arthritis (RA) and in healthy controls (HC)

<p><b>Copyright information:</b></p><p>Taken from "Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4T cells from patients with rheumatoid arthritis"</p><p>Arthritis Research & Therapy 2004;6(6):R567-R577.</p><p>Published online 13 Oct 2004</p><p>PMCID:PMC1064873.</p><p>Copyright © 2004 Yamana et al., licensee BioMed Central Ltd.</p> CD4T cells were purified from peripheral blood mononuclear cells of three RA patients and three HC by positive selection with anti-CD4 antibody. CD4T cells (5 × 10cells in 0.5 ml culture medium with 10% FCS) were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody in the presence or absence of diluted IL-10 concentrations for 36 hours. Culture supernatants were measured for concentrations of IFN-γ by ELISA. IFN-γ production with IL-10 expressed as % IFN-γ production without IL-10. Values are the mean ± standard error of the mean