Expression of hypoxia-inducible factor-1α (HIF-1α) in RA synovium and fibroblast-like synoviocytes under normoxic and hypoxic conditions

<p><b>Copyright information:</b></p><p>Taken from "Oxidation in rheumatoid arthritis"</p><p>Arthritis Research & Therapy 2004;6(6):265-278.</p><p>Published online 13 Oct 2004</p><p>PMCID:PMC1064874.</p><p>Copyright © 2004 BioMed Central Ltd</p> Under normoxic conditions, HIF-1α expression in fresh synovial explants was patchy and confined to some cells in the lining layer. When fresh RA tissue explants were cultured in hypoxic conditions (1% O), nuclear staining for HIF-1α was readily detected in the lining cells. A similar pattern of expression was seen in fibroblast-like synoviocytes where under normoxic conditions no HIF-1α staining was detected (c), whereas under hypoxic conditions intense nuclear staining was seen maximally at 4–6 hours (d). Reproduced, with permission, from [91]