Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma-1

<p><b>Copyright information:</b></p><p>Taken from "Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma"</p><p>BMC Cancer 2005;5():119-119.</p><p>Published online 20 Sep 2005</p><p>PMCID:PMC1266025.</p><p>Copyright © 2005 Lee et al; licensee BioMed Central Ltd.</p>amplifications of and in three samples for each cell line were analysed to determine the relative levels of expression using the BioRad iCycler. All /ratios were close to 1 indicating that there are comparable amounts of both templates in each RNA samples. (B) Dilutions of a single RNA sample for each cell line were also amplified in triplicate with both gene primer sets to confirm that the ratio was consistent regardless of starting template concentration. (C) Triplicate amplifications of , and in three samples for each cell line using the Corbett Rotorgene confirmed the previous result for /and showed a similar result when expression was normalised to a second housekeeping gene