Denaturing gel electrophoresis of RNA transcription using RNA polymerase in the presence of , , , –, (/), (), (/), , and in the absence of inhibitors ()

<p><b>Copyright information:</b></p><p>Taken from "Easily denaturing nucleic acids derived from intercalating nucleic acids: thermal stability studies, dual duplex invasion and inhibition of transcription start"</p><p>Nucleic Acids Research 2005;33(22):7129-7137.</p><p>Published online 23 Dec 2005</p><p>PMCID:PMC1322271.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> is 5′-GCTATGAC (complement to the template strand at +36 to +43); is 3′-GTCATAGC (complement to the non-template strand at +36 to +43). The upper arrow points to the 61mer RNA transcript, and the lower arrow to a 30mer DNA quantification marker. The dashed arrow points to an ∼35mer RNA product. The concentration of inhibitors is 10 µM