Characterization of DNA colonies generated using BTA chemistry by Sybr-Green I staining

<p><b>Copyright information:</b></p><p>Taken from "BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies"</p><p>Nucleic Acids Research 2006;34(3):e22-e22.</p><p>Published online 9 Feb 2006</p><p>PMCID:PMC1363783.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Histograms of colonies average intensity () Before BbvI digestion colonies are double stranded and their length is 347 bp. () After BbvI digestion, colonies are still double stranded and their length is reduced to 43 bp, showing consequently a lower average intensity