Proapoptotic activity of Ukrain is based on L. alkaloids and mediated via a mitochondrial death pathway-3

<p><b>Copyright information:</b></p><p>Taken from "Proapoptotic activity of Ukrain is based on L. alkaloids and mediated via a mitochondrial death pathway"</p><p>BMC Cancer 2006;6():14-14.</p><p>Published online 17 Jan 2006</p><p>PMCID:PMC1379651.</p><p>Copyright © 2006 Habermehl et al; licensee BioMed Central Ltd.</p>24 h with medium or 5, 10 and 50 μg/ml Ukrain. Apoptosis was then determined by flow cytometry using light scatter characteristics (left panel). Alternatively, A3, FADD-deficient and caspase-8-deficient Jurkat cells were treated for 3, 6, 12 and 24 h with medium or 10 μg/ml Ukrain. Apoptosis induction was then quantified by determination of the mitochondrial membrane potential (Δψm) (right panel). Data show induction of specific apoptosis (means ± SD; n ≥ 3). One-way ANOVA was performed using GraphPad InStat version 3.00 for Windows 95, GraphPad Software, San Diego California USA, . . Jurkat A3 as well as FADD-deficient and caspase-8-deficient Jurkat cells were treated for 6, 12 and 24 h with medium or 10 μg/ml Ukrain. Caspase activation was then determined by Western blot analysis of cytosolic extracts with specific antibodies against caspase-3, cleaved caspase-3, PARP and cleaved PARP, respectively. In addition, cells were treated for 3, 6, 12 and 24 h with medium or 10 μg/ml Ukrain and Western blot analysis with specific antibodies against caspase-8 was performed. Data from one representative experiment are shown