RT–PCR detection of mRNA, intact lariat introns, and circular RNAs from either RNase R-digested (+) or mock-digested (−) human skeletal muscle total RNA

<p><b>Copyright information:</b></p><p>Taken from "Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicing"</p><p>Nucleic Acids Research 2006;34(8):e63-e63.</p><p>Published online 8 May 2006</p><p>PMCID:PMC1458517.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The RNA was directly analyzed by 1.0% agarose gel electrophoresis (lanes 1 and 2). The RNA was used for RT–PCR with primers to detect the dystrophin mRNA including exons 7 and 8 (lanes 3 and 4), three lariat introns as indicated (lanes 5–10), and the circular RNAs including multiple exons (lanes 11 and 12). The PCR products were analyzed by native 6.0% PAGE (lanes 3–12). The structures of the lariat intron and circular RNA are schematically represented and arrows indicate the positions of primers used for PCR