Population of open (that is fluorescent) states at 10°C (), 20°C () and 30°C ()

<p><b>Copyright information:</b></p><p>Taken from "The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy"</p><p>Nucleic Acids Research 2006;34(9):2516-2527.</p><p>Published online 10 May 2006</p><p>PMCID:PMC1459409.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> is derived from rate constants measured by FCS (following ). Whereas addition of the first dC–dG pair significantly reduces for all hairpins, the effect of a second dC–dG pair depends strongly on the loop length: for hairpins with loops longer than five residues, is further reduced; for structures with loops of 3, 4 and 5 residues, the effect of the second dC–dG pair is temperature dependent, showing less influence on for shorter loops and lower temperature