Real-time RT–PCR validation of the downregulated predicted miR-124 targets

<p><b>Copyright information:</b></p><p>Taken from "Systematic identification of microRNA functions by combining target prediction and expression profiling"</p><p>Nucleic Acids Research 2006;34(5):1646-1652.</p><p>Published online 20 Mar 2006</p><p>PMCID:PMC1405822.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The mRNA levels for downregulated targets at 4, 24 and 48 h were examined. The expression levels at 24 and 48 h are presented as percentages of that at 4 h. Among the validated targets, the microarray results indicated that genes 8801 and 8992 were first downregulated at 8 h; genes 8763, 3915, 6566, 9341, 10 449 and 10 634 were first downregulated at 16 h; genes 6836, 60 481 and 84 061 were first downregulated at 24 h; genes 27 230 and 55 225 were first downregulated at 32 h (downregulation is defined as at least 50% reduction of gene expression)