Investigation of the selective accumulation of the small immunoproteins L19 and G11 in inflamed limbs of arthritic mice

<p><b>Copyright information:</b></p><p>Taken from "Antibody-mediated delivery of IL-10 inhibits the progression of established collagen-induced arthritis"</p><p>http://arthritis-research.com/content/9/1/R9</p><p>Arthritis Research & Therapy 2007;9(1):R9-R9.</p><p>Published online 29 Jan 2007</p><p>PMCID:PMC1860067.</p><p></p> Arthritic mice were injected with SIP(L19)–Alexa750 (Molecular Probes, Leiden, The Netherlands), SIP(G11)–Alexa750 or the control antibody SIP(HyHEL10)–Alexa750. Near-infrared fluorescence imaging analysis was performed 24 hours after injection of the fluorescence-labelled antibodies. Arrows indicate grade 2 swelling in the front paws of the mice. In a similar experiment, arthritic mice were injected with I-labelled SIP(L19), SIP(G11) or SIP control. Uptake of radio-iodinated antibodies was analysed by phosphorimaging 24 hours after injection. The mouse injected with I-labelled SIP(L19) had an arthritic score of 2 in the left paw, whereas the right paw was classified as grade 1 arthritis. In the mouse injected with I-labelled SIP(G11) the left paw was classified as grade 1 arhritis, whereas the right paw was classified as grade 2 arthritis. In the mouse injected with I-labelled SIP(F16) the left paw was classified as grade 2 arthritis, whereas the right paw as grade 1 arthritis