Reverse transcription initiation efficiency of the wt and J1–J10 transcripts

<p><b>Copyright information:</b></p><p>Taken from "The availability of the primer activation signal (PAS) affects the efficiency of HIV-1 reverse transcription initiation"</p><p></p><p>Nucleic Acids Research 2007;35(5):1649-1659.</p><p>Published online 18 Feb 2007</p><p>PMCID:PMC1865047.</p><p>© 2007 The Author(s).</p> () DNA or tRNA primers were annealed to wt and mutant 1-368 transcripts and extended with P-CTP by the HIV-1 RT enzyme. Extended primers were separated on a denaturing acrylamide gel. The 1/2 tRNA signal represents a half tRNA species that is discussed in detail in the context of . () Relative initiation of reverse transcription efficiency of the wt and mutant transcripts, the wt activity was arbitrarily set at 1. The average and standard deviations of three independent experiments were quantified